FLAG-tag:Article Title: Lactylation-driven nuclear RIG-I promoted by lactate transporter inhibitor suppresses DNA damage repair through inhibiting PARP1 activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies L-lactyl lysine PTM Biolab Cat#PTM-1401RM; RRID:AB_2942013 FLAG tag Sigma Cat#F1804; RRID:AB_262044 HA-tag Sigma Cat#H3663; RRID:AB_262051 Flag M2 affinity gel Sigma Cat#A2220; RRID:AB_10063035 PARP1 CST Cat#46D11 poly/mono-ADP ribose CST Cat#E6F6A pSer386-IRF3 CST Cat#E7J8G γ-H2AX CST Cat#D8O9V c-MYC CST Cat#7C10 α-Tubulin AbClonal Cat#AC012; RRID:AB_2768341 Lamin B1 HuaxingBio Cat#HX1983 GFP-tag Proteintech Cat#50430-2-AP; RRID:AB_11042881 RIG-I Proteintech Cat#67556-1-Ig; RRID:AB_2882770 Ni-NTA Sefinose resin BBI Cat#C600033-0100 RIG-I Abcam Cat#EPR18629 RIG-I Santa Cruz CatSC-376845; RRID:AB_2732794 IPO8 Santa Cruz Cat#SC-398854 IPO8 AbClonal A14679; RRID:AB_2761554 IRF3 Santa Cruz Cat#SC-376445 goat anti-rabbit Licor, IRDye 800CW Cat#926-32211 goat anti-mouse Licor, IRDye 800CW Cat#926-32210 goat anti-mouse (L, HRP) Easybio Cat#BE0105-100 goat anti-rabbit (L, HRP) Easybio Cat#BE0107-100 His-tag MBL Cat#D291-3; RRID: AB_10597733 Bacterial and virus strains DH5α competent E. coli This paper N/A BL21 competent E. coli This paper N/A Chemicals, peptides, and recombinant proteins Syrosingopine TargetMol Cat#TN2252 Nocodazole TargetMol Cat#T2802 Importazole TargetMol Cat#T3445 Olaparib MedChemExpress Cat#HY-10162 Bleomycin MedChemExpress Cat#HY-17565 L-Sodium lactate Sigma Cat#71718 Lactyl-CoA Nafu Cat#SH-NF-1059 Protein G-Sepharose GE Healthcare Cat#17-0618-05 DAPI Beyotime Cat#P0103 protease inhibitor MedChemExpress Cat#HY-K0010 PARP1 MedChemExpress HY-P74652 Critical commercial assays EndoFree Mini Plasmid Kit II TIANGEN Cat#DP118 Annexin V-FITC/PI apoptosis kit Meilunbio Cat#MA0220-2 (Continued on next page) 20 Cell Reports 45, 116854, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Duolink In Situ PLA kit Sigma Cat#DUO92101 L-Lactate Assay kit Abcam Cat#ab65330 CCK-8 kit Meilunbio Cat#MA0218 In vitro PARylation kit GENMED SCIENTIFICS INC. U.S.A GMS50116.1 v.A Deposited data Human Flag-tagged RIG-I lactylation IP-MS in HEK293T This paper PXD058596; https://doi.org/10.6019/PXD058596 Human Flag-tagged RIG-I IP-MS in HEK293T This paper PXD058597; https://doi.org/10.6019/PXD058597 Experimental models: Cell lines HEK293T ATCC https://www.atcc.org/ RIG-I KO HEK293T This paper N/A HeLa ATCC https://www.atcc.org/ A549 ATCC https://www.atcc.org/ RIG-I KO A549 This paper N/A H1299 ATCC https://www.atcc.org/ RIG-I KO A549 rescued with RIG-I WT This paper N/A RIG-I KO A549 rescued with RIG-I 2KR This paper N/A Oligonucleotides sgRNAs for generating cell lines This paper Table S1 Primers for qRT-PCR analysis This paper Table S2 siRNAs for generating PCAF- knockdown cell lines This paper Table S3 siRNAs for generating Importin 8- knockdown cell lines This paper Table S3 Recombinant DNA Flag-RIG-I ΔCTD This paper N/A Flag-RIG-I ΔCARD This paper N/A Flag-RIG-I ΔHCD This paper N/A His-RIG-I WT This paper N/A His-RIG-I 2KR This paper N/A pLVX-Zsgreen-Flag-RIG-I WT This paper N/A pLVX-Zsgreen-Flag-RIG-I 2KR This paper N/A Flag-RIG-I K518R This paper N/A Flag-RIG-I K593R This paper N/A Flag-RIG-I K657R This paper N/A Flag-RIG-I K518/593R This paper N/A Flag-RIG-I K518/657R (2KR) This paper N/A Flag-RIG-I K593/657R This paper N/A Flag-RIG-I K518/593/657R This paper N/A Software and algorithms ImageJ NIH https://imagej.net/ij/ GraphPad Prism v.9.5.0 GraphPad Software http://www.graphpad.com/ Combenefit Combenefit https://sourceforge.net/projects/ combenefit/ Fiji NIH https://imagej.net/software/fiji/ Cell Reports 45, 116854, January 27, 2026 21
Virus:Article Title: Lactylation-driven nuclear RIG-I promoted by lactate transporter inhibitor suppresses DNA damage repair through inhibiting PARP1 activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies L-lactyl lysine PTM Biolab Cat#PTM-1401RM; RRID:AB_2942013 FLAG tag Sigma Cat#F1804; RRID:AB_262044 HA-tag Sigma Cat#H3663; RRID:AB_262051 Flag M2 affinity gel Sigma Cat#A2220; RRID:AB_10063035 PARP1 CST Cat#46D11 poly/mono-ADP ribose CST Cat#E6F6A pSer386-IRF3 CST Cat#E7J8G γ-H2AX CST Cat#D8O9V c-MYC CST Cat#7C10 α-Tubulin AbClonal Cat#AC012; RRID:AB_2768341 Lamin B1 HuaxingBio Cat#HX1983 GFP-tag Proteintech Cat#50430-2-AP; RRID:AB_11042881 RIG-I Proteintech Cat#67556-1-Ig; RRID:AB_2882770 Ni-NTA Sefinose resin BBI Cat#C600033-0100 RIG-I Abcam Cat#EPR18629 RIG-I Santa Cruz CatSC-376845; RRID:AB_2732794 IPO8 Santa Cruz Cat#SC-398854 IPO8 AbClonal A14679; RRID:AB_2761554 IRF3 Santa Cruz Cat#SC-376445 goat anti-rabbit Licor, IRDye 800CW Cat#926-32211 goat anti-mouse Licor, IRDye 800CW Cat#926-32210 goat anti-mouse (L, HRP) Easybio Cat#BE0105-100 goat anti-rabbit (L, HRP) Easybio Cat#BE0107-100 His-tag MBL Cat#D291-3; RRID: AB_10597733 Bacterial and virus strains DH5α competent E. coli This paper N/A BL21 competent E. coli This paper N/A Chemicals, peptides, and recombinant proteins Syrosingopine TargetMol Cat#TN2252 Nocodazole TargetMol Cat#T2802 Importazole TargetMol Cat#T3445 Olaparib MedChemExpress Cat#HY-10162 Bleomycin MedChemExpress Cat#HY-17565 L-Sodium lactate Sigma Cat#71718 Lactyl-CoA Nafu Cat#SH-NF-1059 Protein G-Sepharose GE Healthcare Cat#17-0618-05 DAPI Beyotime Cat#P0103 protease inhibitor MedChemExpress Cat#HY-K0010 PARP1 MedChemExpress HY-P74652 Critical commercial assays EndoFree Mini Plasmid Kit II TIANGEN Cat#DP118 Annexin V-FITC/PI apoptosis kit Meilunbio Cat#MA0220-2 (Continued on next page) 20 Cell Reports 45, 116854, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Duolink In Situ PLA kit Sigma Cat#DUO92101 L-Lactate Assay kit Abcam Cat#ab65330 CCK-8 kit Meilunbio Cat#MA0218 In vitro PARylation kit GENMED SCIENTIFICS INC. U.S.A GMS50116.1 v.A Deposited data Human Flag-tagged RIG-I lactylation IP-MS in HEK293T This paper PXD058596; https://doi.org/10.6019/PXD058596 Human Flag-tagged RIG-I IP-MS in HEK293T This paper PXD058597; https://doi.org/10.6019/PXD058597 Experimental models: Cell lines HEK293T ATCC https://www.atcc.org/ RIG-I KO HEK293T This paper N/A HeLa ATCC https://www.atcc.org/ A549 ATCC https://www.atcc.org/ RIG-I KO A549 This paper N/A H1299 ATCC https://www.atcc.org/ RIG-I KO A549 rescued with RIG-I WT This paper N/A RIG-I KO A549 rescued with RIG-I 2KR This paper N/A Oligonucleotides sgRNAs for generating cell lines This paper Table S1 Primers for qRT-PCR analysis This paper Table S2 siRNAs for generating PCAF- knockdown cell lines This paper Table S3 siRNAs for generating Importin 8- knockdown cell lines This paper Table S3 Recombinant DNA Flag-RIG-I ΔCTD This paper N/A Flag-RIG-I ΔCARD This paper N/A Flag-RIG-I ΔHCD This paper N/A His-RIG-I WT This paper N/A His-RIG-I 2KR This paper N/A pLVX-Zsgreen-Flag-RIG-I WT This paper N/A pLVX-Zsgreen-Flag-RIG-I 2KR This paper N/A Flag-RIG-I K518R This paper N/A Flag-RIG-I K593R This paper N/A Flag-RIG-I K657R This paper N/A Flag-RIG-I K518/593R This paper N/A Flag-RIG-I K518/657R (2KR) This paper N/A Flag-RIG-I K593/657R This paper N/A Flag-RIG-I K518/593/657R This paper N/A Software and algorithms ImageJ NIH https://imagej.net/ij/ GraphPad Prism v.9.5.0 GraphPad Software http://www.graphpad.com/ Combenefit Combenefit https://sourceforge.net/projects/ combenefit/ Fiji NIH https://imagej.net/software/fiji/ Cell Reports 45, 116854, January 27, 2026 21
Recombinant:Article Title: Lactylation-driven nuclear RIG-I promoted by lactate transporter inhibitor suppresses DNA damage repair through inhibiting PARP1 activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies L-lactyl lysine PTM Biolab Cat#PTM-1401RM; RRID:AB_2942013 FLAG tag Sigma Cat#F1804; RRID:AB_262044 HA-tag Sigma Cat#H3663; RRID:AB_262051 Flag M2 affinity gel Sigma Cat#A2220; RRID:AB_10063035 PARP1 CST Cat#46D11 poly/mono-ADP ribose CST Cat#E6F6A pSer386-IRF3 CST Cat#E7J8G γ-H2AX CST Cat#D8O9V c-MYC CST Cat#7C10 α-Tubulin AbClonal Cat#AC012; RRID:AB_2768341 Lamin B1 HuaxingBio Cat#HX1983 GFP-tag Proteintech Cat#50430-2-AP; RRID:AB_11042881 RIG-I Proteintech Cat#67556-1-Ig; RRID:AB_2882770 Ni-NTA Sefinose resin BBI Cat#C600033-0100 RIG-I Abcam Cat#EPR18629 RIG-I Santa Cruz CatSC-376845; RRID:AB_2732794 IPO8 Santa Cruz Cat#SC-398854 IPO8 AbClonal A14679; RRID:AB_2761554 IRF3 Santa Cruz Cat#SC-376445 goat anti-rabbit Licor, IRDye 800CW Cat#926-32211 goat anti-mouse Licor, IRDye 800CW Cat#926-32210 goat anti-mouse (L, HRP) Easybio Cat#BE0105-100 goat anti-rabbit (L, HRP) Easybio Cat#BE0107-100 His-tag MBL Cat#D291-3; RRID: AB_10597733 Bacterial and virus strains DH5α competent E. coli This paper N/A BL21 competent E. coli This paper N/A Chemicals, peptides, and recombinant proteins Syrosingopine TargetMol Cat#TN2252 Nocodazole TargetMol Cat#T2802 Importazole TargetMol Cat#T3445 Olaparib MedChemExpress Cat#HY-10162 Bleomycin MedChemExpress Cat#HY-17565 L-Sodium lactate Sigma Cat#71718 Lactyl-CoA Nafu Cat#SH-NF-1059 Protein G-Sepharose GE Healthcare Cat#17-0618-05 DAPI Beyotime Cat#P0103 protease inhibitor MedChemExpress Cat#HY-K0010 PARP1 MedChemExpress HY-P74652 Critical commercial assays EndoFree Mini Plasmid Kit II TIANGEN Cat#DP118 Annexin V-FITC/PI apoptosis kit Meilunbio Cat#MA0220-2 (Continued on next page) 20 Cell Reports 45, 116854, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Duolink In Situ PLA kit Sigma Cat#DUO92101 L-Lactate Assay kit Abcam Cat#ab65330 CCK-8 kit Meilunbio Cat#MA0218 In vitro PARylation kit GENMED SCIENTIFICS INC. U.S.A GMS50116.1 v.A Deposited data Human Flag-tagged RIG-I lactylation IP-MS in HEK293T This paper PXD058596; https://doi.org/10.6019/PXD058596 Human Flag-tagged RIG-I IP-MS in HEK293T This paper PXD058597; https://doi.org/10.6019/PXD058597 Experimental models: Cell lines HEK293T ATCC https://www.atcc.org/ RIG-I KO HEK293T This paper N/A HeLa ATCC https://www.atcc.org/ A549 ATCC https://www.atcc.org/ RIG-I KO A549 This paper N/A H1299 ATCC https://www.atcc.org/ RIG-I KO A549 rescued with RIG-I WT This paper N/A RIG-I KO A549 rescued with RIG-I 2KR This paper N/A Oligonucleotides sgRNAs for generating cell lines This paper Table S1 Primers for qRT-PCR analysis This paper Table S2 siRNAs for generating PCAF- knockdown cell lines This paper Table S3 siRNAs for generating Importin 8- knockdown cell lines This paper Table S3 Recombinant DNA Flag-RIG-I ΔCTD This paper N/A Flag-RIG-I ΔCARD This paper N/A Flag-RIG-I ΔHCD This paper N/A His-RIG-I WT This paper N/A His-RIG-I 2KR This paper N/A pLVX-Zsgreen-Flag-RIG-I WT This paper N/A pLVX-Zsgreen-Flag-RIG-I 2KR This paper N/A Flag-RIG-I K518R This paper N/A Flag-RIG-I K593R This paper N/A Flag-RIG-I K657R This paper N/A Flag-RIG-I K518/593R This paper N/A Flag-RIG-I K518/657R (2KR) This paper N/A Flag-RIG-I K593/657R This paper N/A Flag-RIG-I K518/593/657R This paper N/A Software and algorithms ImageJ NIH https://imagej.net/ij/ GraphPad Prism v.9.5.0 GraphPad Software http://www.graphpad.com/ Combenefit Combenefit https://sourceforge.net/projects/ combenefit/ Fiji NIH https://imagej.net/software/fiji/ Cell Reports 45, 116854, January 27, 2026 21
Protease Inhibitor:Article Title: Lactylation-driven nuclear RIG-I promoted by lactate transporter inhibitor suppresses DNA damage repair through inhibiting PARP1 activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies L-lactyl lysine PTM Biolab Cat#PTM-1401RM; RRID:AB_2942013 FLAG tag Sigma Cat#F1804; RRID:AB_262044 HA-tag Sigma Cat#H3663; RRID:AB_262051 Flag M2 affinity gel Sigma Cat#A2220; RRID:AB_10063035 PARP1 CST Cat#46D11 poly/mono-ADP ribose CST Cat#E6F6A pSer386-IRF3 CST Cat#E7J8G γ-H2AX CST Cat#D8O9V c-MYC CST Cat#7C10 α-Tubulin AbClonal Cat#AC012; RRID:AB_2768341 Lamin B1 HuaxingBio Cat#HX1983 GFP-tag Proteintech Cat#50430-2-AP; RRID:AB_11042881 RIG-I Proteintech Cat#67556-1-Ig; RRID:AB_2882770 Ni-NTA Sefinose resin BBI Cat#C600033-0100 RIG-I Abcam Cat#EPR18629 RIG-I Santa Cruz CatSC-376845; RRID:AB_2732794 IPO8 Santa Cruz Cat#SC-398854 IPO8 AbClonal A14679; RRID:AB_2761554 IRF3 Santa Cruz Cat#SC-376445 goat anti-rabbit Licor, IRDye 800CW Cat#926-32211 goat anti-mouse Licor, IRDye 800CW Cat#926-32210 goat anti-mouse (L, HRP) Easybio Cat#BE0105-100 goat anti-rabbit (L, HRP) Easybio Cat#BE0107-100 His-tag MBL Cat#D291-3; RRID: AB_10597733 Bacterial and virus strains DH5α competent E. coli This paper N/A BL21 competent E. coli This paper N/A Chemicals, peptides, and recombinant proteins Syrosingopine TargetMol Cat#TN2252 Nocodazole TargetMol Cat#T2802 Importazole TargetMol Cat#T3445 Olaparib MedChemExpress Cat#HY-10162 Bleomycin MedChemExpress Cat#HY-17565 L-Sodium lactate Sigma Cat#71718 Lactyl-CoA Nafu Cat#SH-NF-1059 Protein G-Sepharose GE Healthcare Cat#17-0618-05 DAPI Beyotime Cat#P0103 protease inhibitor MedChemExpress Cat#HY-K0010 PARP1 MedChemExpress HY-P74652 Critical commercial assays EndoFree Mini Plasmid Kit II TIANGEN Cat#DP118 Annexin V-FITC/PI apoptosis kit Meilunbio Cat#MA0220-2 (Continued on next page) 20 Cell Reports 45, 116854, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Duolink In Situ PLA kit Sigma Cat#DUO92101 L-Lactate Assay kit Abcam Cat#ab65330 CCK-8 kit Meilunbio Cat#MA0218 In vitro PARylation kit GENMED SCIENTIFICS INC. U.S.A GMS50116.1 v.A Deposited data Human Flag-tagged RIG-I lactylation IP-MS in HEK293T This paper PXD058596; https://doi.org/10.6019/PXD058596 Human Flag-tagged RIG-I IP-MS in HEK293T This paper PXD058597; https://doi.org/10.6019/PXD058597 Experimental models: Cell lines HEK293T ATCC https://www.atcc.org/ RIG-I KO HEK293T This paper N/A HeLa ATCC https://www.atcc.org/ A549 ATCC https://www.atcc.org/ RIG-I KO A549 This paper N/A H1299 ATCC https://www.atcc.org/ RIG-I KO A549 rescued with RIG-I WT This paper N/A RIG-I KO A549 rescued with RIG-I 2KR This paper N/A Oligonucleotides sgRNAs for generating cell lines This paper Table S1 Primers for qRT-PCR analysis This paper Table S2 siRNAs for generating PCAF- knockdown cell lines This paper Table S3 siRNAs for generating Importin 8- knockdown cell lines This paper Table S3 Recombinant DNA Flag-RIG-I ΔCTD This paper N/A Flag-RIG-I ΔCARD This paper N/A Flag-RIG-I ΔHCD This paper N/A His-RIG-I WT This paper N/A His-RIG-I 2KR This paper N/A pLVX-Zsgreen-Flag-RIG-I WT This paper N/A pLVX-Zsgreen-Flag-RIG-I 2KR This paper N/A Flag-RIG-I K518R This paper N/A Flag-RIG-I K593R This paper N/A Flag-RIG-I K657R This paper N/A Flag-RIG-I K518/593R This paper N/A Flag-RIG-I K518/657R (2KR) This paper N/A Flag-RIG-I K593/657R This paper N/A Flag-RIG-I K518/593/657R This paper N/A Software and algorithms ImageJ NIH https://imagej.net/ij/ GraphPad Prism v.9.5.0 GraphPad Software http://www.graphpad.com/ Combenefit Combenefit https://sourceforge.net/projects/ combenefit/ Fiji NIH https://imagej.net/software/fiji/ Cell Reports 45, 116854, January 27, 2026 21
Plasmid Preparation:Article Title: Lactylation-driven nuclear RIG-I promoted by lactate transporter inhibitor suppresses DNA damage repair through inhibiting PARP1 activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies L-lactyl lysine PTM Biolab Cat#PTM-1401RM; RRID:AB_2942013 FLAG tag Sigma Cat#F1804; RRID:AB_262044 HA-tag Sigma Cat#H3663; RRID:AB_262051 Flag M2 affinity gel Sigma Cat#A2220; RRID:AB_10063035 PARP1 CST Cat#46D11 poly/mono-ADP ribose CST Cat#E6F6A pSer386-IRF3 CST Cat#E7J8G γ-H2AX CST Cat#D8O9V c-MYC CST Cat#7C10 α-Tubulin AbClonal Cat#AC012; RRID:AB_2768341 Lamin B1 HuaxingBio Cat#HX1983 GFP-tag Proteintech Cat#50430-2-AP; RRID:AB_11042881 RIG-I Proteintech Cat#67556-1-Ig; RRID:AB_2882770 Ni-NTA Sefinose resin BBI Cat#C600033-0100 RIG-I Abcam Cat#EPR18629 RIG-I Santa Cruz CatSC-376845; RRID:AB_2732794 IPO8 Santa Cruz Cat#SC-398854 IPO8 AbClonal A14679; RRID:AB_2761554 IRF3 Santa Cruz Cat#SC-376445 goat anti-rabbit Licor, IRDye 800CW Cat#926-32211 goat anti-mouse Licor, IRDye 800CW Cat#926-32210 goat anti-mouse (L, HRP) Easybio Cat#BE0105-100 goat anti-rabbit (L, HRP) Easybio Cat#BE0107-100 His-tag MBL Cat#D291-3; RRID: AB_10597733 Bacterial and virus strains DH5α competent E. coli This paper N/A BL21 competent E. coli This paper N/A Chemicals, peptides, and recombinant proteins Syrosingopine TargetMol Cat#TN2252 Nocodazole TargetMol Cat#T2802 Importazole TargetMol Cat#T3445 Olaparib MedChemExpress Cat#HY-10162 Bleomycin MedChemExpress Cat#HY-17565 L-Sodium lactate Sigma Cat#71718 Lactyl-CoA Nafu Cat#SH-NF-1059 Protein G-Sepharose GE Healthcare Cat#17-0618-05 DAPI Beyotime Cat#P0103 protease inhibitor MedChemExpress Cat#HY-K0010 PARP1 MedChemExpress HY-P74652 Critical commercial assays EndoFree Mini Plasmid Kit II TIANGEN Cat#DP118 Annexin V-FITC/PI apoptosis kit Meilunbio Cat#MA0220-2 (Continued on next page) 20 Cell Reports 45, 116854, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Duolink In Situ PLA kit Sigma Cat#DUO92101 L-Lactate Assay kit Abcam Cat#ab65330 CCK-8 kit Meilunbio Cat#MA0218 In vitro PARylation kit GENMED SCIENTIFICS INC. U.S.A GMS50116.1 v.A Deposited data Human Flag-tagged RIG-I lactylation IP-MS in HEK293T This paper PXD058596; https://doi.org/10.6019/PXD058596 Human Flag-tagged RIG-I IP-MS in HEK293T This paper PXD058597; https://doi.org/10.6019/PXD058597 Experimental models: Cell lines HEK293T ATCC https://www.atcc.org/ RIG-I KO HEK293T This paper N/A HeLa ATCC https://www.atcc.org/ A549 ATCC https://www.atcc.org/ RIG-I KO A549 This paper N/A H1299 ATCC https://www.atcc.org/ RIG-I KO A549 rescued with RIG-I WT This paper N/A RIG-I KO A549 rescued with RIG-I 2KR This paper N/A Oligonucleotides sgRNAs for generating cell lines This paper Table S1 Primers for qRT-PCR analysis This paper Table S2 siRNAs for generating PCAF- knockdown cell lines This paper Table S3 siRNAs for generating Importin 8- knockdown cell lines This paper Table S3 Recombinant DNA Flag-RIG-I ΔCTD This paper N/A Flag-RIG-I ΔCARD This paper N/A Flag-RIG-I ΔHCD This paper N/A His-RIG-I WT This paper N/A His-RIG-I 2KR This paper N/A pLVX-Zsgreen-Flag-RIG-I WT This paper N/A pLVX-Zsgreen-Flag-RIG-I 2KR This paper N/A Flag-RIG-I K518R This paper N/A Flag-RIG-I K593R This paper N/A Flag-RIG-I K657R This paper N/A Flag-RIG-I K518/593R This paper N/A Flag-RIG-I K518/657R (2KR) This paper N/A Flag-RIG-I K593/657R This paper N/A Flag-RIG-I K518/593/657R This paper N/A Software and algorithms ImageJ NIH https://imagej.net/ij/ GraphPad Prism v.9.5.0 GraphPad Software http://www.graphpad.com/ Combenefit Combenefit https://sourceforge.net/projects/ combenefit/ Fiji NIH https://imagej.net/software/fiji/ Cell Reports 45, 116854, January 27, 2026 21
Staining:Article Title: TGF-β mediates epigenetic control of innate antiviral responses and SIV reservoir size
Article Snippet: Staining was performed as per the BD staining protocol with BD Phosflow Fix Buffer I (BD, cat. no. 557870) and BD Phosflow Perm Buffer III (BD, cat. no. 558050). .. The staining panel included: Live/dead APC-Cy7, BD cat. no. 565388; CD3 BUV805, BD cat. no. 612895, Clone UCHT1; CD4 BV605, BioLegend cat. no. 317438, Clone OKT4; CD8 BUV737, BD cat. no. 564629, Clone SK1; CD45RA BV650, BioLegend cat. no. 304136, Clone HI100; CD27 BUV615, BD cat. no. 751685, Clone O323; CCR7 PE-CF594, BD cat. no. 562381, Clone 2-L1-A; HDAC11 PE, Santa Cruz cat. no. sc-390737PE, Clone C-5; HDAC1 FITC, Santa Cruz cat. no. sc-81598FITC, Clone 10E-2; HLA-DR BV786, BD Biosciences cat. no. 564041, Clone G46-6; CD19 BUV395, BD Biosciences cat. no. 563549, Clone SJ25C1; CD14 BV570, BioLegend cat. no. 301832, Clone M5E2; CD16 BUV661, BD Biosciences cat. no. 741693, Clone B73.1; CD56 PE-Cy5, BD Biosciences cat. no. 555517, Clone B159. ..
Article Title: TGF-β mediates epigenetic control of innate antiviral responses and SIV reservoir size
Article Snippet: .. The staining panel comprised Live/dead BV510, Life Technologies cat. no. L34957 ; CD3 BUV615, BD cat. no. 612992, Clone UCHT1; CD4 BV605, BioLegend cat. no. 317438, Clone OKT4; CD8 BUV737, BD cat. no. 564629, Clone SK1; CD45RA BV650, BioLegend cat. no. 304136, Clone HI100; CD27 APC-eFluor 780, eBioscience cat. no. 47027942, Clone O323; CCR7 BUV563, BD cat. no. 741317, Clone 3D12; pSMAD2(pS465/pS467)/pSMAD3 (pS423/pS425) PE-CF594, BD cat. no. 562697, Clone O72-670; HDAC11 PE, Santa Cruz cat. no. sc-390737PE, Clone C-5; and HIV-1 (p24) APC, Medimabs cat. no. MM-0289-APC, Clone 28B7. ..
Article Title: TGF-β mediates epigenetic control of innate antiviral responses and SIV reservoir size.
Article Snippet: Staining was performed as per the BD staining protocol with BD Phosflow Fix Buffer I (BD, cat. no. 557870) and BD Phosflow Perm Buffer III (BD, cat. no. 558050). .. The staining panel included: Live/dead APC-Cy7, BD cat. no. 565388; CD3 BUV805, BD cat. no. 612895, Clone UCHT1; CD4 BV605, BioLegend cat. no. 317438, Clone OKT4; CD8 BUV737, BD cat. no. 564629, Clone SK1; CD45RA BV650, BioLegend cat. no. 304136, Clone HI100; CD27 BUV615, BD cat. no. 751685, Clone O323; CCR7 PE-CF594, BD cat. no. 562381, Clone 2-L1-A; HDAC11 PE, Santa Cruz cat. no. sc-390737PE, Clone C-5; HDAC1 FITC, Santa Cruz cat. no. sc-81598FITC, Clone 10E-2; HLA-DR BV786, BD Biosciences cat. no. 564041, Clone G46-6; CD19 BUV395, BD Biosciences cat. no. 563549, Clone SJ25C1; CD14 BV570, BioLegend cat. no. 301832, Clone M5E2; CD16 BUV661, BD Biosciences cat. no. 741693, Clone B73.1; CD56 PE-Cy5, BD Biosciences cat. no. 555517, Clone B159. ..
Immunohistochemistry:
Slice Preparation:
Incubation:
Saline:
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Santa Cruz Biotechnology is a verified supplier 